Results: Nucleotide sequences analysis indicated that the isolated and cloned K9-Flag sequence length was 1 380 bp. The isolated K9 sequence was 100% homology with K9 gene previously registered in GenBank. 结果:PCR分离、克隆的K9-Flag序列全长1380bp,核酸序列分析显示克隆的K9基因与GenBank中己经登记的K9基因序列100%同源,引入的Flag序列完全正确;
? During the senescence of flag leaf in winter wheat, the sequence of decline of photosynthetic pigment content and pigment-protein combined degree was that carotenoid ( car) was earlier than chlorophyll a ( chla), and chla was earlier than chlorophyll b ( chlb). 小麦旗叶衰老过程中,光合色素含量与色素&蛋白质结合牢固程度降低的顺序为:类胡萝卜素早于叶绿素a,叶绿素a早于叶绿素b;
Construct the eukaryotic expression vector of including Flag tag sequence of envelope glycoprotein of NDV, and prepared the NDV pseudotyped virus. 成功构建了含Flag标签序列的NDV囊膜糖蛋白基因的真核表达质粒,制备出了NDV-F/HN假型病毒。